Lecture 20

Long-Distance Transport in Plants

Transport in Plants

Two types

Short-Distance Transport

Long-Distance Transport

Short-Distance Transport

Cellular

Cell to cell

Involves osmosis, diffusion, active transport

Long-Distance Transport

One plant part to another (leaf to root)

Transport of sap in xylem and phloem

Involves bulk flow

Bulk flow

Definition: Bulk flow is the movement of a fluid driven by pressure 

Diffusion, osmosis and active transport at the cellular level create pressures

Four Transport Functions 

Absorption of water and minerals by roots

Transport of xylem sap (transport of water and minerals by bulk flow in the hollow [dead] pipes of the xylem)

Control of transpiration (loss of water by evaporation from the stomata of leaves) 

Translocation of phloem sap (the transport of sugars by bulk flow from the leaves to other parts of the plant)

Overview of Water and Mineral Transport 

5steps

Entering the root

Crossing the cortex

Crossing the selective barrier at the endodermis (enter the stele )

Entering the xylem

Flowing up the xylem

Absorption of Water and Minerals by Roots
Entering the Root

Travel by two pathways

Symplast: the cytoplasm within cells (inside cell membrane)

Apoplast: spaces between cells (includes cell walls)

Symplast is living: Apoplast is nonliving

Absorption of Water and Minerals by Roots
Entering the Symplast of Root

Root hairs, mycorrhizae, epidermal cell membrane

Root hairs at the growing tips of root (the primary root)

Water enters by osmosis 

Minerals by diffusion or active transport 

Absorption of Water and Minerals by Roots
Entering the Apoplast of Root

Most common route

Cell walls are hydrophilic (sucks water in)

Water and dissolved minerals enter together

Soak into space between epidermal cells into cortex apoplast

Absorption of Water and Minerals by Roots
Crossing the Cortex
The Apoplastic route

Water and minerals flow together as a solution

Sucked along by the hydrophilic cell walls

Continuous film from soil water to stele

Cannot enter stele by apoplast

Absorption of Water and Minerals by Roots
Crossing the Cortex
the Symplastic route

Cell to cell by plasmodesmata

Cytoplasmic extensions joining cells

Continuous pathway of cytoplasm 

Can enter symplast anywhere in cortex from apoplast

Large surface area for entry to cortex

Selective uptake of minerals entering the symplast

Water follows by osmosis

Absorption of Water and Minerals by Roots
Crossing the Endodermis

Endodermis is barrier for apoplastic water and minerals

Prevents leakage from the stele 

Space between cells of epidermis is blocked 

Casparian strip, a belt of suberin (a waxy material) 

All must enter via the symplast

Selective uptake of minerals occurs 

( e.g. K+ is taken up Na+ is not)

20.1.3 Absorption of Water and Minerals by Roots: Entering the Xylem

-the xylem which consists of dead cells ( lacking cytoplasm and cell wall= no protoplast) are part of the apoplast

-hence the last step is to leave the symplast of the endodermis to enter the apoplast of the xylem

-minerals are transferred out of the symplast of the endodermis and living parenchyma of the stele by both diffusion and active transport creating a hypertonic solution in the apoplast which pulls the water into the apoplast by osmosis

-water (carrying dissolved minerals) in the apoplast now freely enters the vessel elements and tracheids of the xylem